Antimicrobial Activity of Chlorhexidine Gluconate 0.05% against Bacterial Contamination of Dental Unit Drains

Research journal of Anna.P., Pradono, S., S. Mangundjaja, A. Djais

Department of Oral and Maxillofacial Surgery, Department of Oral Biology

Faculty of Dentistry, University of Indonesia

Abstract

Most general practice dental unit drains have become a potential source of infection as a result most of the water used during routine dental procedures has bacterial contamination above the level considered acceptable for safe drinking water. The use of anti-microbial agents to eliminate microorganisms that cause infectious diseases has been discussed for several years. One of the widely used anti-microbial agents to reduce the bacterial load in the mouth is Chlorhexidin.

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Research Objectives

The purpose of this study was to determine the sensitivity of Chlorhexidine gluconate 0.05% to bacterial load in dental unit drains.

Methods

Chlorhexidine gluconate 0.05% known as CH solution was examined in vitro to inhibit bacterial growth by determining the zone of inhibition (agar diffusion method), The bacterial microorganisms tested were bacteria isolated from the

Dental Unit drains at the Community Dental Health Service. The microorganisms tested were Pseudomonas aeruginosa, Staphylococcus aureus, Fusobacterium spp, Diptheroid spp Propionibacterium, Micrococcus, Streptococcus spp. The data obtained was done descriptively.

Results

Showed CHX solution was most effective against Pseudomonas aeruginosa (inhibition zone 2.20 mm); Staphylococcus aureus (inhibition zone 4.30 mm), Fusobacterium spp (inhibition zone 4.50 mm, Diptheroid spp (inhibition zone 2.80 mm) Propionibacterium (inhibition zone 3.00 mm), Micrococcus spp (inhibition zone 3.90 Streptococcus spp (inhibition zone 2.50 mm).

A separate test was performed on samples taken from untreated Dental Unit System samples were plates in blood agar medium and incubated in anaerobic chamber for 72 hours. Colony Forming Units found were > 60,000 CFU/ml.

Conclusion

Chlorhexidine gluconate 0.05% has anti-microbial activity against bacteria isolated from Dental Unit Drains. It is recommended to disinfect Dental Unit Drains with CHX 0.05% on a regular basis